The discovery of human embryonic stem cells (hESCs) has dramatically increased the tools available to medical scientists interested in regenerative medicine. However, direct injection of hESCs, and cells differentiated from hESCs, into living organisms has thus far been hampered by significant cell death, teratoma formation, and host immune rejection. Understanding the in vivo hESC behavior after transplantation requires novel imaging techniques to longitudinally monitor hESC localization, proliferation, and viability.
Wilson Kitchener and colleagues from Stanford University School of Medicine cooked a 10 minutes movie in which they show how in each stem cell, transcription and translation of luciferase into bioactive light-emitter was detected with sensitive, noninvasive instrumentation (CCD cameras from Caliper) directly in alive, sleeping animals.
- Kitchener, W., Yu, J., Lee, A., Wu, J. (2008). In vitro and in vivo bioluminescence reporter gene imaging of human embryonic stem cells. Journal of Visualized Experiments, 2008(14) DOI: 10.3791/740