- detection of beta-galactosidase activity;
- targeting of amide protons of expressed proteins;
- expression of natural iron homeostasis proteins such as transferrin, ferritin, MagA.
Nuclear Magnetic Reporters
With the advantage of tomographical data set and detailed anatomical information, magnetic resonance imaging (MRI) features an unsurpassed space resolution (micrometers!) in the field of in vivo imaging. Conversely, imaging of optical reporters like luciferase and GFP, still suffers from short-sighted resolution, often requiring longer post-mortem analysis (i.e. microscopy-slides) to identify and deconvolve photon emission sources from in vivo datasets. From this "topological" standpoint it is easy to understand the growing awareness and demand of new 'super-paramagnetic"'' reporter genes to be detected according to their 'relaxivity'. NMR gene expression strategies thus far introduced in recent literature include: