Basal expression from inducible transgenes

ResearchBlogging.orgOf course, a critical drawback of inducible transgene expression is the amount of basal expression (leakage) responsible to making noise in the discrimination of specific transgene patterns analysis (and even, to reduce potentially harmful effects of transgene).

The Australian team of from Stuart M Pitson (University of Adelaide) by incorporating AU-rich mRNA destabilizing elements (ARE) into the 3′ untranslated region (UTR) of inducible constructs, demonstrated that this modification minimized sphingosine kinase 1 (SK1) basal expression from a Tet-inducible vector with only a slight decrease in the maximum level of fully induced SK1. This approach, might improve signal-to-noise ratio and should also be applicable to other inducible expression systems.

On the other hand, if your transgene is a reporter gene, sometimes a minimal basal level expression would be appealing to monitor physiological patterns that could be increased or decreased by your treatments. Isn't it?

Duyen Pham, Paul Moretti, Gregory Goodall, Stuart Pitson (2008). Attenuation of leakiness in doxycycline-inducible expression via incorporation of 3′ AU-rich mRNA destabilizing elements BioTechniques, 45 (2), 155-162 DOI: 10.2144/000112896

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