just another luciferase-cell line followed in vivo

At JoVE is available another step-by-step protocol movie regarding Endothelial cells derived from stem cells for treatment of ischemia (hindlimb). The movie protocol includes:

  • Differentiation of murine ESCs into endothelial cells;
  • Construction of the Double Fusion Reporter Gene and Lentiviral Transduction;
  • Transplantation of ESC-derived endothelial cells to the ischemic hindlimb;
  • Bioluminescence imaging of ESC-derived endothelial cells in vivo.

From: Jove.com