At JoVE is available another step-by-step protocol movie regarding Endothelial cells derived from stem cells for treatment of ischemia (hindlimb). The movie protocol includes:
- Differentiation of murine ESCs into endothelial cells;
- Construction of the Double Fusion Reporter Gene and Lentiviral Transduction;
- Transplantation of ESC-derived endothelial cells to the ischemic hindlimb;
- Bioluminescence imaging of ESC-derived endothelial cells in vivo.