Parsley, lips and luciferases

The 2005 Burbelo's method to reverse profile antibody responses to single or multiple antigens (LIPS, Luciferase Immunoprecipitation Systems) is now available as a video protocol on Jove. The overall approach involves expressing chimeric genes encoding probe antigens fused to Renilla luciferase (RLuc). Crude RLuc-antigen extracts are then mixed with sera in examen and A/G beads to quantify and profile interacting antibodies present in sera: when the RLuc-antigen fusion become immobilized on the A/G beads, antigen-specific antibody is quantitated by washing the beads and adding coelenterazine lucifease substrate and measuring light production. Luciferase il like parsley: you can add it in most recipes.

Btw, does anyone has ever got a luciferase antibody that really works?