At JoVE is available another step-by-step protocol movie regarding Endothelial cells derived from stem cells for treatment of ischemia (hindlimb). The movie protocol includes:
- Differentiation of murine ESCs into endothelial cells;
- Construction of the Double Fusion Reporter Gene and Lentiviral Transduction;
- Transplantation of ESC-derived endothelial cells to the ischemic hindlimb;
- Bioluminescence imaging of ESC-derived endothelial cells in vivo.
Darwin was not aware of reporter genes. Sometimes you broke your head in engineering, designing, conceptualizing the perfect molecular assay to monitor a biological process. You ask yourself whether a genetically-based approach would be better than a probe-based approach with fluorophores or whatever. You are so trapped in your commitment to hack the original system with that smart molecular sensor, that you miss to consider simplicity.
|from Flickr: (C) DEAN|
Bernhard Kräutler and co-workers at the University of Innsbruck in Austria, discovered that because of chlorophyll breakdown, there is a blue luminescence in ripening bananas under UV light. No molecular approaches to estimate the degree of ripening, just observation (at UV light). Interestingly, not all animals see in the same spectral range, and a fruit color determines which species can see it ripening (birds and insects can see ultraviolet light, primates cannot). Next time I'm going to the grocery, I'll bring the transilluminator.
Simone Moser, Thomas Müller, Marc-Olivier Ebert, Steffen Jockusch, Nicholas J. Turro, Bernhard Kräutler (2008). Blue Luminescence of Ripening Bananas Angewandte Chemie International Edition, 47 (46), 8954-8957 DOI: 10.1002/anie.200803189
1) your original draft was initially rejected from your PI citing Samuel Johnson:
Your manuscript is both good and original; but the part that is good is not original, and the part that is original is not good.2) your second proof was rejected from the [Science|Nature|Cell] editorial board
Your manuscript is of insufficient immediate interest to our broader readership to justify its publication.3) the third proof was rejected from that anonymous reviewer of the Journal of Molecular Proctology:
The results are chancy and they lack any theoretical interest