Comments on Reportergene: Where do you express your transgene?

Thanks sparc for yours always appropriate comments...

2007-11-29T10:23:00.000+01:00

Thanks sparc for yours always appropriate comments. It's true, the mouse of Kinzel is designed to easily express your preferred gene (thanks also to RMCE, that maybe I will include in a post like "How do you place your tg"). I don't mentioned it explicitily, because I'm trying to tailor the blog mainly on coincised transversal informations on reporter genes.
Rosa26 is patented, and I agree with your conclusion. The BPR3 icon it is a good suggestion that I'm going to review. Thanks again for your attention to details.

BTW, you may consider to add the BPR3 icon to your...

2007-11-28T06:10:00.000+01:00

BTW, you may consider to add the BPR3 icon to your posts when you discuss peer reviewed research. Since post labeled with BPR3 will be aggregated in the future this will improve the visibility of your blog. You will find the guidelines for the use of the BPR3 icon at
http://bpr3.org/

One point you do not explicitly mention in your po...

2007-11-28T06:07:00.000+01:00

One point you do not explicitly mention in your post is the fact that the authors engineered the ß-actin locus in a way one can exchange the transgene to be expressed by recombinase mediated cassette exchange (RMCE). Thus beside using this mouse line as a reporter for tissue specific Cre acitivity it can be used to easily engineer lines that express a gene of interest in a tissue specific and/or temporally controlled manner.
BTW, one may question why the authors work on an alternative for rosa26 driven trangenes. Of course it will be useful to have an alternative locus that may outperform rosa26 in certain applications. However, to my best knowledge the rosa26 locus is patented. Thus, researchers of for profit institutions may have problems to use it without licensing.