Quantification of neutralizing antibodies to human type I interferons using division-arrested frozen cells carrying an interferon-regulated reporter-gene

Quantification of neutralizing antibodies to human type I interferons using division-arrested frozen cells carrying an interferon-regulated reporter-gene

Improvement of neutralizing antibodies (NAbs) to interferons (IFNs) can scale back the scientific response to IFN remedy. As present cell-based assays for quantifying NAbs have limitations, a extremely delicate and reproducible assay was developed, utilizing division-arrested frozen human U937 cells transfected with the luciferase <em>reportergene</em> managed by an IFN-responsive chimeric promoter, which permits IFN exercise […]